DNA for every particular person was extracted from both fin or muscle tissue from every fish that had been saved at −80 °C utilizing Qiagen DNeasy extraction columns, quantified utilizing a Qubit 2.0, and run on a 1% agarose gel to evaluate DNA high quality. DNA was then sequenced on the Illumina NovaSeq 6000 with a 550 bp insert dimension (Subsequent Technology Sequencing Platform, College of Bern). To this information, we added Illumina HiSeq 3000 information sequenced from one Coregonus sp. “Balchen” (ENA accession: GCA_902810595.1; now re-classified as C. steinmanni31) from Lake Thun (Switzerland) that was beforehand used to shine and validate the Alpine whitefish reference genome meeting47.
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