Metabolic adaptation to vitamin auxotrophy by leaf-associated bacteria

Strains and development assays

All At-LSPHERE strains used on this examine had been beforehand printed [30]. All development assays had been carried out at 28 °C for At-LSPHERE strains and 37 °C for E. coli [53] strains. The turbidity of shake flask cultures was decided by measuring the optical density at 595 nm (=“OD₆₀₀”) in semi-micro cuvettes (Bio-Greiner) utilizing a Biophotometer Plus (Eppendorf). Samples had been diluted as acceptable to maintain the OD readings within the linear vary. For batch cultures with steady OD monitoring for 96 effectively plates (TPP flat backside), Tecan Infinite M200 Professional was used at 1 mm amplitude orbital shaking.

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