They developed a neat option to observe oil accumulation. Via genetic engineering, they created Arabidopsis vegetation by which a inexperienced fluorescent protein is all the time hooked up to a protein known as oleosin. Oleosin solely accumulates on the floor of lipid droplets. It varieties a part of the membrane surrounding these oil-storage compartments inside cells to assist stabilize them. If a pattern of plant tissue—leaf, stem, or seed—accommodates lipid droplets, they stand out as little inexperienced dots beneath a fluorescence microscope.
“We handled our Arabidopsis vegetation with a mutagen to attempt to set off mutations that may improve oil accumulation,” Xu mentioned, utilizing the fluorescence method to establish strains with extra and/or larger inexperienced dots.
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