To additional validate our proteomics information, we examined one of many unstable complexes, RNA polymerase III, which was recognized in each 8+15L and 16L and may very well be biochemically purified from whole cell extracts53. We calculated a near two-fold discount (49%) within the abundance of RNA polymerase III subunits in 16L cells, though the identical whole quantities of protein lysates from 16L and Sc cells had been used for the pull-down experiment (Supplementary Fig. 8a). Collectively, these information point out that when subunits have dissociated from destabilized complicated, they’re rapidly degraded or type insoluble aggregates.
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