Certainly, our protein aggregation assay confirmed that every one the examined alternative traces in addition to the F1 hybrid diploids had a considerably increased proportion of cells with Hsp104 foci at an early time-point (45 min) and took longer to dissolve all aggregates in comparison with the S. cerevisiae management (Fig. 1c and Supplementary Fig. 1a). At 180 min after the temperature shift, Hsp104 foci had been undetectable in most S. cerevisiae management cells, however each the alternative traces and F1 hybrid diploids exhibited vital retention of aggregates (Fig. 1d and Supplementary Fig. 2). These outcomes corroborate our transcriptome information that proteostasis is perturbed in cells carrying overseas chromosomes. Amongst our diploid alternative traces, S. cerevisiae cells carrying S. bayanus var. uvarum chromosomes 5 and seven (5+7L), 8 and 15 (8+15L), and 16 (16L) exhibited the slowest adaptive kinetics, suggesting that these traces may show probably the most extreme intrinsic proteotoxic stress. Since we had beforehand established that the 5+7L alternative line additionally suffers from mitochondrial-nuclear incompatibility29, we centered our subsequent experimental analyses on the 8+15L and 16L traces.
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