Metabolic adaptation to vitamin auxotrophy by leaf-associated bacteria
The method for focus and manufacturing fee of S2 led to biologically practical focus vary (~5 mM; Supplementary Fig. 10). On the receiving finish, the expansion charges for S2 had been estimated as follows
$$mu _{2,,Pressure} = mu _{Pressure,,max } ast Scaling_factor ast frac{{left[ {S_2} right]}}{{left[ {S_2} right] + K_{S_2}}}$$
For simulations during which the auxotrophs and prototrophs had been equally environment friendly, the Scaling_factor parameter was set to 1/3. In an effort to make auxotrophs extra environment friendly, the scaling issue was set to 1 for auxotrophs and stored at 1/3 for prototrophs. The sensitivity for this parameter was examined by setting it in vary from 1/2 to 2. The focus of every pressure was simulated as follows:
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