Metabolic adaptation to vitamin auxotrophy by leaf-associated bacteria

Media

The medium base was a phosphate buffer (2.4 g/l Okay₂HPO₄, 2.08 g/l NaH₂PO₄·2H₂O) with mineral salts (1.62 g/l NH₄Cl, 0.2 g/l MgSO₄·7H₂0). Glucose was added at 20 mM and pyruvate at 40 mM. For all media parts, 10x shares had been ready, dissolved in ddH₂O and filter sterilized. Nutritional vitamins had been supplemented when indicated within the following concentrations: D-pantothenic acid hemi calcium salt 1.05 µM, biotin 0.41 µM, riboflavin 1.06 µM, thiamine · HCl 1.19 µM, pyridoxal · HCl 0.98 µM, p-amino benzoic acid 1.09 µM, cobalamine 0.14 µM, lipoic acid 0.24 µM, niacin 1.22 µM, folic acid 0.23 µM. All 20 proteinogenic L-amino acids had been supplemented at 0.1 mM every, besides L-tryptophan and L-tyrosine had been supplemented at 0.05 mM. 1000x shares had been ready for all different nutritional vitamins and amino acids apart from riboflavin and tryptophan for which 100x shares had been ready.

Comments

0 comments