Hybrid sterility is commonly noticed upon mating between two species. To look at the impact of intrinsic proteotoxic stress on meiosis, we perturbed the proteostasis of alternative traces with the identical delicate dosage of GdA earlier than sporulation after which measured sporulation charges (see “Strategies”). Two alternative traces (7L and 13L) had been excluded from this assay since they already exhibited extreme sporulation defects on account of mitochondrial-nuclear incompatibility28. Among the many 9 examined alternative traces, six offered considerably increased sensitivity to the perturbation than the parental traces and exhibited considerably diminished sporulation (Fig. 2c). In keeping with our protein aggregation information, the 8+15L and 16L traces confirmed probably the most extreme defects in each mitosis and meiosis amongst all examined alternative traces. These outcomes show that though hybrid cells carrying overseas chromosome(s) solely show weak or delicate incompatibility, the health defects might be simply aggravated by delicate environmental perturbations which might be tolerable to wild-type cells.
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