If 26S proteasomes are probably the most essential degradation equipment controlling protein complicated homeostasis in our alternative traces, we anticipated hybrid cells to show extreme progress defects when their proteasomes are compromised, even below regular progress situations. We constructed heterozygous deletion mutants of Rpn6, an important lid part of the 26S proteasome, from our 8+15L and 16L traces, and measured their health at 23 and 28 °C. Heterozygous RPN6/rpn6Δ mutation solely partially compromised the exercise of proteasomes and had delicate impacts on the health of Sc cells at each temperatures. In distinction, the RPN6/rpn6Δ mutants of the 16L and eight+15L traces revealed a big health defect at 28 °C (Fig. 4d and Supplementary Fig. 8c).
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